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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Cell Rep. 2016 Sep 27;17(1):69–85. doi: 10.1016/j.celrep.2016.08.073

Figure 7.

Figure 7

NAD+ deficiency impairs mitochondrial sirtuin function. (A–B) NAMPT inhibition caused selective mitochondrial protein hyperacetylation (n=4 biological replicates from two independent blots; 1-way ANOVA with Tukey post-hoc test). (C–D) NAMPT inhibition caused only modest changes in mitochondrial protein succinylation (n=6 biological replicates from three independent blots; 1-way ANOVA with Tukey post-hoc test). (E) NAD+ deficiency impaired SIRT3 activity (n=6/group; 2-tailed, unpaired t-test) but not SIRT5 activity (F; n=15–16/group; 2-tailed, unpaired t-test). Graphs depict mean + S.E.M. (B, D–F) (** p < .01; *** p < .001).