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. 2016 Sep 26;12(5):4013–4020. doi: 10.3892/ol.2016.5191

Figure 2.

Figure 2.

Expression of Mfn2 was downregulated following TNF-α-induced myotube atrophy of C2C12 cells. (A) Undifferentiated C2C12 cells, (B) C2C12 cells differentiated for 7 days, (C) C2C12 cells differentiated for 7 days in the presence of 2.5 ng/ml TNF-α and (D) C2C12 cells differentiated for 7 days in the presence of 5 ng/ml TNF-α. (E) Length of myotube formed by differentiated C2C12 cells. (F) Transverse diameter of myotube formed by differentiated C2C12 cells. (G) Protein expression levels of Mfn2, COX IV, Cyto C and β-actin in C2C12 cells and differentiated C2C12 cells treated with or without TNF-α. **P<0.01, ***P<0.001 vs. the control (0 ng/ml TNF-α). Mfn2, mitofusin-2; TNF-α, tumor necrosis factor-α; COX-IV, cytochrome c oxidase subunit IV; Cyto C, cytochrome c.