Figure 4.

Expression of Mfn2 inhibited TNF-α-induced fragmentation of mitochondria. (A) Electron microscopy of C2C12 cells cultured in differentiation medium for 7 days. (B) C2C12 cells were differentiated for 7 days in the presence of 5 ng/ml TNF-α. (C) C2C12 cells were infected with Mfn2 lentivirus, and then cultured in differentiation medium containing 5 ng/ml TNF-α 7 days. The red arrows indicate mitochondria in cells. (D) Measurement of the length of mitochondria in C2C12 cells. **P<0.01. Mfn2, mitofusin-2; TNF-α, tumor necrosis factor-α.