Table III.
PCR primers used to amplify genes correlated with cancer pathways in qPCR reaction.
| Genes | Primers sequence (5′-3′) | Product size, bp | Annealing temperature, °C |
|---|---|---|---|
| GAPDH | F: AGCCACATCGCTCAGACA | 66 | 60.0 |
| R: GCCCAATACGACCAAATCC | 63.0 | ||
| MET | F: TTCTGACCGAGGGAATCAT | 82 | 60.0 |
| R: CCTTCACTTCGCAGGCAG | |||
| MMP9 | F: GAACCAATCTCACCGACAGG | 67 | 63.0 |
| R: GCCACCCGAGTGTAACCATA | |||
| PDGFA | F: CCCCTGCCCATTCGGAGGAAGAG | 227 | 60.0 |
| R: TTGGCCACCTTGACGCTGCGGTG | |||
| SERPINE1 | F: CTCTCTCTGCCCTCACCAA | 183 | 60.0 |
| R: GAAACTGTCTGAACATGTCG |
F-forward; R-reverse; qPCR, quantitative polymerase chain reaction.