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Vit. C regulates the expression and activation of proteins related to the apoptosis and autophagy pathways. AGS cells were treated with 0.5 or 1.5 mM Vit. C for 4 h. (A) The expression of pro-caspase-3 and cleaved caspase-3 and (B) the expression of LC3 I and LC3 II were measured in vitamin C-treated and untreated cells. β-actin was used as a loading control. Data are representative of three independent experiments. Vit., vitamin; LC3, light chain 3.
