Skip to main content
. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Immunol. 2016 Sep 19;197(8):3302–3314. doi: 10.4049/jimmunol.1601219

Figure 2.

Figure 2

BMP9 increases neutrophil recruitment to LPS-stimulated endothelial cells. Neutrophils were perfused over confluent endothelial monolayers treated with BMP9 (16 h; 5 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Total neutrophil recruitment to (A) BOECs and (C) HPAECs. Neutrophil behaviours of rolling, arrest and transmigration were expressed as a percentage of total recruitment to (B) BOECs and (D) HPAECs. Data was collected from 3 experiments, and error bars correspond to ± SEM. *p<0.05 **p<0.01 *** p<0.001 ****p<0.0001 compared with untreated cells and #p<0.05 ##p<0.01 ###0.001 ####p.0001 compared with LPS-stimulated cells. BOECs were transfected with siALK1 and siALK2 and treated with BMP9 (16 hours; 5 ng/ml) prior to the addition of LPS (4 hours; 100 ng/ml). (E) Representative images (100x) showing surface arrested neutrophils (SA) as bright phase and transmigrated (TM) neutrophils as dark phase. (F) Total recruitment to siALK1 and siALK2 transfected BOECs. Data was collected from 3-4 experiments, and error bars correspond to ± SEM, **p<0.01.