Skip to main content
. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Immunol. 2016 Sep 19;197(8):3302–3314. doi: 10.4049/jimmunol.1601219

Figure 4.

Figure 4

BMP9-mediated upregulation of E-selectin and VCAM-1 is concentration dependent. Blood outgrowth endothelial cells (BOECs) were treated with 5 different concentrations of BMP9 (ranging from 0-5 ng/ml) for 16 h prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to assess surface expression of (A) E-selectin (FITC-conjugated anti-human E-selectin), (B) VCAM-1 (PE-Cy5-conjugated anti-human VCAM-1) and (C) ICAM-1 (APC-conjugated anti-human ICAM-1). Forward scatter and side scatter gating was applied to the BOEC population. Histograms are representative of 3 experiments. BMP9 dose response curves show median fluorescence intensity (MFI) as fold change relative to untreated BOECs. Error bars correspond to ± SEM, n=3, *p<0.05.