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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Prenat Diagn. 2016 Jul 25;36(9):812–822. doi: 10.1002/pd.4862

Figure 5.

Figure 5

Expression of S100 calcium-binding protein A7 (S100A7) was down regulated in the three trisomies. (A) In euploid samples (e.g., 21 wks), strong S100A7 immunoreactivity was detected in the stromal cores of anchoring villi (AV); the cell columns (CC) of emigrating cytotrophoblasts stained variably and weakly. (B–D) (B) Little to no staining was observed in comparable regions from T13 (18 wks), (C) T18 (20 wks) and (D) T21 (22 wks) placentas. When present, antibody binding was detected only in association with the villous stromal compartments. (E) Immunoblotting showed S100A7 expression in normal pregnancy and uniform downregulation in T13, T18 and T21. (F) Quantitation of expression normalized to actin and relative to control euploid samples showed statistically significant (*) decreases in the 3 trisomies (p= 0.02). Size bars = 100 μm; Eu, euploid; CK7, cytokeratin 7; GA, gestational age; arrows indicate direction of cytotrophoblast invasion.