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(A-E) Differentiating mESCs immunostained for TuJ (beta-III tubulin) on different days of co-culture with PA6 cells. (F) TuJ expression quantified using daily fluorescent intensity measurements show continuous increase in protein content in culture. (G) An independent adaptive thresholding method applied to TuJ-stained images shows increasing TuJ expression over the culture period. Scale bar: 100 μm. * indicates p<0.05.
