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. 2016 Nov 10;11(11):e0166384. doi: 10.1371/journal.pone.0166384

Table 1. National meningitis data quality and completeness indicators, Burkina Faso, 2011–2013.

2011 2012 2013 Total
N (%) N (%) N (%) N (%)
Districts submitting case-based surveillance data 57/63 (90) 63/63 (100) 63/63 (100) 63/63 (100)
Districts submitting CSF specimens 57/63 (90) 63/63 (100) 63/63 (100) 63/63 (100)
Suspected meningitis cases with a LPa 2,766 (97) 6,302 (97) 2,758 (97) 11,826 (97)
    CSFs assessed for appearance 2,417 (87) 5,723 (91) 2,585 (94) 10,725 (91)
    CSFs with a Gram stainb 2,508 (91) 5,626 (89) 2,460 (89) 10,594 (90)
    CSFs tested by cytology 1217 (44) 2,473 (39) 1,271 (46) 4,961 (42)
    CSFs tested at a national laboratoryc,d 1,242 (45) 2,379 (38) 1,836 (67) 5,457 (46)
    CSFs tested by latex 1,146 (41) 1,205 (19) 513 (19) 2,864 (24)
    CSFs tested by culture 722 (26) 1,552 (25) 637 (23) 2,911 (25)
    CSFs cultured but found to be contaminatede 190/722 (26) 275/1,552 (18) 107/637 (17) 572/2,911 (20)
    CSFs tested by rt-PCR 1,133 (41) 1,396 (22) 1,704 (62) 4,233 (36)

Abbreviations: CSF, cerebrospinal fluid; LP, lumbar puncture; rt-PCR, real-time polymerase chain reaction.

a 1,166 (10%) of CSFs were tested by latex only, 742 (6%) by culture only, 2,017 (17%) by rt-PCR only, 482 (4%) by latex and culture, 529 (4%) by latex and rt-PCR, 1,000 (8%) by culture and rt-PCR, and 687 (6%) by all three methods. 5,203 (44%) were not tested by any of the three methods.

b Possible reasons why an LP may not reach a district laboratory for gram staining: difficulty with specimen transport, geographic inaccessibility of certain health centers, insufficient transport media

c Defined as being tested by culture and/or rt-PCR

d Possible reasons why an LP may not reach a national reference laboratory: insufficient transport media, insufficient quantity of CSF that was entirely used for Gram staining and cytology.

e 447 (78%) of CSFs cultured but found to be contaminated were also tested by rt-PCR.