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. 2016 Nov 10;11(11):e0165531. doi: 10.1371/journal.pone.0165531

Fig 2. IFC discriminates between viable and non-viable pollen populations.

Fig 2

Pollen of three different species, cucumber (green), sweet pepper (blue) and tomato (red) were inactivated by heat. The effect of the heat treatment on pollen viability was analysed by IFC and classical FDA staining. A, AmphaSoft dot plots and histograms at 0.5 MHz of fresh (left) and heat-treated (right) tomato pollen populations; B, Temperature-dependent decrease of pollen viability detected by IFC at 12 MHz, * p = < 0.05; C, Correlation of IFC data at 12 MHz with classical FDA staining.