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. 2016 Nov 10;11(11):e0165960. doi: 10.1371/journal.pone.0165960

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© 2016 Moraes et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — A-B, H. brasiliensis (population from Paraty, Brazil); C, H. brasiliensis (population from Ubatuba, Brazil); D-E, H. equitans; F-G, H. villosa; H, Heterotaxis chromosomes showing pericentromeric DAPI⁺ bands; I-J, H. valenzuelana. A, D, F and I: CMA (yellow)/DAPI (blue) banding. H: DAPI⁺ bands. B, E, G and J: in situ hybridization using 45S rDNA (green) and 5S rDNA (red). C: 5S rDNA (red). Arrows in A, F and I indicate CMA⁰/DAPI⁻or CMA⁺/DAPI⁻bands. Arrows and arrowheads in B, E, G and J show 45S rDNA and 5S rDNA, respectively. Arrowheads in C show 5S rDNA and arrows indicated the CMA^–/DAPI⁻chromosome gap. Detail in A, F and I indicate chromosomes with CMA⁺/DAPI⁻bands and in B, G and J, the same chromosomes with 45S rDNA sites (green). Detail in J shows also the chromosome pair with 5S rDNA sites (red). Chromosomes in the inserts in A, B, I and J could be selected from an alternative metaphase. Insets in C and D show the chromosome with CMA^–/DAPI⁻gap. Bars in H and J represent 10 m.