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. 2016 Nov 10;11(11):e0166352. doi: 10.1371/journal.pone.0166352

Fig 2. Activation of the NRF2 transcription factor reporter system by different agents of radical stress or KEAP1 modifier.

Fig 2

(A) Cell cultures of HEK293-hNQ cells were treated for 24 h with tBHQ (3, 6, 12, 18, 25, 37, 50, 75, 100 [μM]), CSE (0.04, 0.08, 0.16, 0.32, 0.63, 1.25, 2.5, 5, 10 [% (v/v)]) or Paraquat (25, 50, 75, 100, 125, 150, 200, 250, 300 [μM]). Luciferase activity was expressed as % of untreated samples (Control). (B) Cultures of HEK293-hNQ and HEK293-hNQ-del cells were transfected with positive and negative control siRNAs and lysed 48 h later for the determination of luciferase activity. Treatment of these cells with the NRF2 activator tBHQ at 50μM was performed for 24h. All results were normalized to untreated HEK293-hNQ samples. Values are represented as means of independent biological experiments ± SD (n = 6).