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. 2016 Nov 10;11(11):e0165460. doi: 10.1371/journal.pone.0165460

Fig 1. Cell proliferation at the SVZ in a rat model of SAH.

Fig 1

Coronal sections of forebrain were immunostained with anti-Ki67 antibody (red, proliferating cell), anti-nestin antibody (green, NSC) and Hoechst 33342 (blue, nucleus).The level of cell proliferation in the SVZ was determined by Ki67/ Hoechst 33342 co-staining. (A)-(T): photomicrograph showing the distribution of Ki67+ (A, E, I, M, and Q) and nestin+ (B, F, J, N, and R) signals and as merged images (D, H, L, P, and T) in the SVZ of animals at different SAH time course (1, 3, 5, and 7 days after SAH) and sham control. Scale bar = 50 μm. (U)-(X): Images of Ki67+ /nestin+ NSCs in the SVZ on day 7 post SAH showing Ki67 (U) and nestin (V) immunoreactivity separately or as merged image (X). Scale bar = 7.5 μm. (Y): Percentage of NSCs (Nestin+ of Hoechst+ cells) in the SVZ. (Z): Percentage of proliferating cells (Ki67+ of Hoechst+ cells) in the SVZ. Means ± SD, n = 6 for each time-point. *, P < 0.05; ***, P < 0.001, one way ANOVA with Dunnett's multiple comparisons test.