. 2016 Nov 10;11(11):e0165702. doi: 10.1371/journal.pone.0165702

Table 2. Primers utilized in PCR amplification of Piroplasmida mitochondrial genomes.

Amplicon	Forward Primer	Reverse Primer
MT Genome Fragment 1^a	`GGAAGTGGWACWGGWTGGAC`	`ACTTTGAACACACTGCTCG`
MT Genome Fragment 2^a	`AGGCATGCAATACCGAACAGG`	`AAGGTACGCCRGGGATAACAGG`
MT Genome Fragment 3^a	`AAGGTATGGTGAGACGACATGG`	`CTTAACCCAACTCACGTACC`
*cox1*^b^, ^c	`GGAAGTGGWACWGGWTGGAC`	`TTCGGTATTGCATGCCTTG`
*cytb*^b	`TTAGTGAAGGAACTTGACAGGT`	`CGGTTAATCTTTCCTATTCCTTACG`
*cox3*^b	`ACTGTCAGCTAAAACGTATC`	`ACAGGATTAGATACCCTGG`
cox3 (Babesia microti group)^b	`CTCGATATTAATCTTAAAGTACAGGAC`	`ACTCATATCTATTACCACTATAGGC`

^aPrimers were designed based on sequences of previously reported related Piroplasmida mitochondrial genomes. Three primer sets were utilized for the amplification of a near-full length mitochondrial genome for the majority of species (5 out of 7) characterized in this study. For additional primers used see S1–S7 Tables.

^bAfter sequencing of the mitochondrial genomes was complete, primers were designed in highly conserved regions for amplification of partial cox1 and full length cytb and cox3 in all species, and are recommended for amplification of these genes in future studies^.

^cRecommended primer set for amplification of cox1 for phylogenetic analysis