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. 2016 Nov 10;11(11):e0166363. doi: 10.1371/journal.pone.0166363

Fig 2. Inactivation of Rb leads to decreased expression of MEG3 and re-expression inhibits proliferation in A549 cells.

Fig 2

(A) Relative expression of MEG3 was determined by qPCR in NHBE and A549 (p16 deletion) lung cancer cells. (B) A549 cells were transfected with either a plasmid encoding human MEG3 or empty vector and viable cell number was determined at 48, 72 and 96 h Results are shown as mean ± S.D. for results from at least three independent experiments. (C&D) Distribution of the cells in the G1, S and G2/M phases of the cell cycle after 48 h by propidium iodide (PI) staining and flow cytometry. (E&F) Apoptotic rates were measured by the presence of PI and Annexin V positive cells after 48 h by flow cytometry. *p<0.05.