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. 2016 Nov 10;11(11):e0166363. doi: 10.1371/journal.pone.0166363

Fig 3. Treatment of A549 & SK-MES-1 cells with palbociclib increases expression of MEG3 in a dose-dependent manner.

Fig 3

Relative expression of MEG3 was determined by qPCR in (A) A549 and (B) SK-MES-1 cells treated with increasing concentrations of palbociclib for 48 h. The relative abundance of MEG3 in cells treated with vehicle was set as 1. (C) Representative Western blot demonstrating phospho-Rb and total Rb in A549 and SK-MES-1 cells treated with increasing concentrations of palbociclib for 48 h. (D) Densitometry analysis of the ratio of phosphor-Rb/total Rb from A549 and SK-MES-1 cells treated with increasing concentrations of palbociclib for 48 h. Results are shown as mean ± S.D. for results from at least three independent experiments. *p<0.05.