Table 2. Descriptive data of all studies for the meta-analysis regarding SCSA, SCD test, TUNEL assay and Comet assay as tools for measure sperm DNA fragmentation.
A. Characteristics of studies included in meta-analysis on SCSA | |||||||
Study | Pro-/ retrospective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
Boe-Hanson 2006 | Prospective | Mixed | Unclear | IVF | <27 | 139 | CP |
ICSI | <27 | 47 | CP | ||||
Bungum 2007 | Prospective | Mixed | Pre-wash | IVF | <30 | 388 | CP*, LB |
ICSI | <30 | 223 | CP*, LB | ||||
Check 2005 | Prospective | Previously failed ART | Unclear | ICSI | <30 | 106 | CP, OP* |
Gandini 2004 | Prospective | Mixed | Pre-wash | IVF | <27** | 12 | P*, LB |
ICSI | <27** | 22 | P*, LB | ||||
Guerin 2005 | Unclear | Unclear | Unclear | IVF/ICSI | <30 | 100 | P |
Larson-Cook 2003 | Retrospective | Not specified | Pre-wash | IVF/ICSI | <27 | 89 | CP, P* |
Lin 2008 | Pro- and retrospective | Not specified | Unclear | IVF | <9, <27* | 137 | CP |
ICSI | <9, <27* | 86 | CP | ||||
Micinski 2009 | Prospective | Not specified | Unclear | ICSI | <15 | 60 | P |
Niu 2011 | Prospective | Mixed | Post-wash | IVF | <27 | 256 | CP, OP* |
Oleszczuk 2016 | Retrospective | Mixed | Pre-wash | IVF | <10, <20* | 1117 | P*, LB |
ICSI | <10, <20* | 516 | P*, LB | ||||
Payne 2005 | Prospective | Not specified | Pre-wash | IVF/ICSI | <27 | 98 | P |
Simon 2014 | Prospective | Not specified | Pre-wash | IVF/ICSI | <27 | 96 | P |
Speyer 2010 | Pro- and retrospective | Mixed | Pre-wash | IVF | <19, <30* | 192 | P |
ICSI | <19, <30* | 155 | P | ||||
Virro 2004 | Pro- and retrospective | Not specified | Pre-wash | IVF/ICSI | <30 | 249 | OP |
B. Characteristics of studies included in meta-analysis on SCD test | |||||||
Study | Pro-/retro-spective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
Anifandis 2015 | Prospective | Not specified | Pre-wash | IVF/ICSI | <35 | 156 | CP*, OP |
Ni 2014 | Prospective | Not specified | Unclear | IVF | <30 | 855 | CP*, LB |
ICSI | <30 | 227 | CP*, LB | ||||
Muriel 2006 | Prospective | Not specified | Post-wash | IVF/ICSI | <32.8 | 85 | P |
Wang 2014 | Prospective | Male infertility | Pre- and post-wash | ICSI | <30 | 45 | CP |
Yilmaz 2010 | Prospective | Male infertility | Pre-wash | ICSI | <30 | 60 | P |
C. Characteristics of studies included in meta-analysis on TUNEL assay | |||||||
Study | Pro-/retro-spective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
Benchaib 2007 | Prospective | Not specified | Post-wash | IVF | <15 | 88 | CP, OP* |
ICSI | <15 | 234 | CP, OP* | ||||
Borini 2006 | Prospective | Mixed | Post-wash | IVF | <10 | 82 | CP |
ICSI | <10 | 50 | CP | ||||
Esbert 2011 | Prospective | Mixed | Pre-wash | IVF | <36 | 77 | CP |
Frydman 2008 | Prospective | Mixed | Pre-wash | IVF | <35 | 117 | CP, OP*, LB |
Henkel 2004 | Prospective | Not specified | Pre-wash | IVF | <36.5 | 167 | P |
Huang 2005 | Retrospective | Not specified | Post-wash | IVF | <4, <10, <15* | 217 | P |
ICSI | <4, <10, <15* | 86 | P | ||||
Ozmen 2007 | Prospective | Not specified | Post-wash | ICSI | <4, <10* | 42 | CP |
Seli 2004 | Prospective | Not specified | Post-wash | IVF/ICSI | <20 | 49 | CP |
Simon 2014 | Prospective | Not specified | Post-wash | IVF/ICSI | <10 | 224 | P |
D. Characteristics of studies included in meta-analysis on Comet assay | |||||||
Study | Pro-/retro-spective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
Simon 2010 | Prospective | Not specified | Pre- and post*-wash | IVF | <44, <56* | 224 | CP*, LB |
ICSI | <44, <56* | 127 | CP*, LB | ||||
Simon 2011 | Prospective | Male infertility | Pre- and post*-wash | IVF | <42, <52* | 70 | P |
Simon 2011 | Prospective | Mixed | Pre- and post*-wash | IVF | <42, <52* | 73 | P |
Simon 2014 | Prospective | Not specified | Unclear | IVF/ICSI | <82 | 229 | P |
* used for meta-analysis
** threshold determined by authors of this review. CP: clinical pregnancy; DFI: DNA fragmentation index; LB: live birth; OP: ongoing pregnancy; P: pregnancy; SCD: sperm chromatin dispersion; SCSA: sperm chromatin structure assay.