Table 2. Descriptive data of all studies for the meta-analysis regarding SCSA, SCD test, TUNEL assay and Comet assay as tools for measure sperm DNA fragmentation.
| A. Characteristics of studies included in meta-analysis on SCSA | |||||||
| Study | Pro-/ retrospective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
| Boe-Hanson 2006 | Prospective | Mixed | Unclear | IVF | <27 | 139 | CP |
| ICSI | <27 | 47 | CP | ||||
| Bungum 2007 | Prospective | Mixed | Pre-wash | IVF | <30 | 388 | CP*, LB |
| ICSI | <30 | 223 | CP*, LB | ||||
| Check 2005 | Prospective | Previously failed ART | Unclear | ICSI | <30 | 106 | CP, OP* |
| Gandini 2004 | Prospective | Mixed | Pre-wash | IVF | <27** | 12 | P*, LB |
| ICSI | <27** | 22 | P*, LB | ||||
| Guerin 2005 | Unclear | Unclear | Unclear | IVF/ICSI | <30 | 100 | P |
| Larson-Cook 2003 | Retrospective | Not specified | Pre-wash | IVF/ICSI | <27 | 89 | CP, P* |
| Lin 2008 | Pro- and retrospective | Not specified | Unclear | IVF | <9, <27* | 137 | CP |
| ICSI | <9, <27* | 86 | CP | ||||
| Micinski 2009 | Prospective | Not specified | Unclear | ICSI | <15 | 60 | P |
| Niu 2011 | Prospective | Mixed | Post-wash | IVF | <27 | 256 | CP, OP* |
| Oleszczuk 2016 | Retrospective | Mixed | Pre-wash | IVF | <10, <20* | 1117 | P*, LB |
| ICSI | <10, <20* | 516 | P*, LB | ||||
| Payne 2005 | Prospective | Not specified | Pre-wash | IVF/ICSI | <27 | 98 | P |
| Simon 2014 | Prospective | Not specified | Pre-wash | IVF/ICSI | <27 | 96 | P |
| Speyer 2010 | Pro- and retrospective | Mixed | Pre-wash | IVF | <19, <30* | 192 | P |
| ICSI | <19, <30* | 155 | P | ||||
| Virro 2004 | Pro- and retrospective | Not specified | Pre-wash | IVF/ICSI | <30 | 249 | OP |
| B. Characteristics of studies included in meta-analysis on SCD test | |||||||
| Study | Pro-/retro-spective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
| Anifandis 2015 | Prospective | Not specified | Pre-wash | IVF/ICSI | <35 | 156 | CP*, OP |
| Ni 2014 | Prospective | Not specified | Unclear | IVF | <30 | 855 | CP*, LB |
| ICSI | <30 | 227 | CP*, LB | ||||
| Muriel 2006 | Prospective | Not specified | Post-wash | IVF/ICSI | <32.8 | 85 | P |
| Wang 2014 | Prospective | Male infertility | Pre- and post-wash | ICSI | <30 | 45 | CP |
| Yilmaz 2010 | Prospective | Male infertility | Pre-wash | ICSI | <30 | 60 | P |
| C. Characteristics of studies included in meta-analysis on TUNEL assay | |||||||
| Study | Pro-/retro-spective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
| Benchaib 2007 | Prospective | Not specified | Post-wash | IVF | <15 | 88 | CP, OP* |
| ICSI | <15 | 234 | CP, OP* | ||||
| Borini 2006 | Prospective | Mixed | Post-wash | IVF | <10 | 82 | CP |
| ICSI | <10 | 50 | CP | ||||
| Esbert 2011 | Prospective | Mixed | Pre-wash | IVF | <36 | 77 | CP |
| Frydman 2008 | Prospective | Mixed | Pre-wash | IVF | <35 | 117 | CP, OP*, LB |
| Henkel 2004 | Prospective | Not specified | Pre-wash | IVF | <36.5 | 167 | P |
| Huang 2005 | Retrospective | Not specified | Post-wash | IVF | <4, <10, <15* | 217 | P |
| ICSI | <4, <10, <15* | 86 | P | ||||
| Ozmen 2007 | Prospective | Not specified | Post-wash | ICSI | <4, <10* | 42 | CP |
| Seli 2004 | Prospective | Not specified | Post-wash | IVF/ICSI | <20 | 49 | CP |
| Simon 2014 | Prospective | Not specified | Post-wash | IVF/ICSI | <10 | 224 | P |
| D. Characteristics of studies included in meta-analysis on Comet assay | |||||||
| Study | Pro-/retro-spective cohort | Infertility | Semen | Treatment | Cut-off DFI (%) | No. cycles | Outcome |
| Simon 2010 | Prospective | Not specified | Pre- and post*-wash | IVF | <44, <56* | 224 | CP*, LB |
| ICSI | <44, <56* | 127 | CP*, LB | ||||
| Simon 2011 | Prospective | Male infertility | Pre- and post*-wash | IVF | <42, <52* | 70 | P |
| Simon 2011 | Prospective | Mixed | Pre- and post*-wash | IVF | <42, <52* | 73 | P |
| Simon 2014 | Prospective | Not specified | Unclear | IVF/ICSI | <82 | 229 | P |
* used for meta-analysis
** threshold determined by authors of this review. CP: clinical pregnancy; DFI: DNA fragmentation index; LB: live birth; OP: ongoing pregnancy; P: pregnancy; SCD: sperm chromatin dispersion; SCSA: sperm chromatin structure assay.