Figure 6.

lft1 MO injections in wild-type and LZoep embryos. Lateral views of 18–20 somite stage LZoep embryos injected with a control MO (a) or the lft1 MO (b). lft1-injected embryos developed without apparent defects, but were often slightly delayed compared with uninjected controls. Dorsal views of pitx2 expression in lft1 MO-injected wild-type (c) or LZoep (d,e) embryos at the 18 somite stage. Anterior is up; left (L) and right (R) are indicated. (f) Bar graph representing pitx2 expression in MO-injected embryos. Genotype of injected embryos is listed first, followed by the injected MO. The ntla MO results from figure 4 are shown here to aid in comparison with the lft1 MO results. Expression categories and colour codes are described in figure 1. (g) Statistical significance of experiments conducted as described in figure 3.