Figure 5.

Temperature-sensitive period influencing heterotaxy coincides with in utero embryonic development. Adjacent piecharts show proportion of hermaphrodites with any heterotaxy and stacked bar shows proportion of each class of heterotaxy. (a) Pre-gastrulation-stage embryos cultured continuously to adulthood at 20°C (n = 672). (b) Pre-gastrulation-stage embryos upshifted from 20°C to 25°C and cultured to adulthood at 25°C (n = 300). (c) Pre-gastrulation-stage embryos cultured continuously to adulthood at 25°C (n = 475). (d) Pre-gastrulation-stage embryos downshifted from 25°C to 20°C and cultured to adulthood at 20°C (n = 251). Sample size is pooled from n ≥ 2 replicates per condition when p > 0.05 using Fisher's exact test. Fisher's exact test was also used to compare all experimental conditions.