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. 2016 Dec 19;371(1710):20150406. doi: 10.1098/rstb.2015.0406

Figure 2.

Figure 2.

pfkp but not pitrm1 Xenopus laevis morphants develop organ situs defects. (a) Genes in CNV interval. Source: USCS genome browser (https://genome.ucsc.edu/). (b) RT-PCR results demonstrate that pfkp is expressed throughout critical L–R patterning stages. ODC, ornithine decarboxylase. (c) pfkp knockdown in 2-cell embryos causes heterotaxy in Xenopus. Situs defects in st. 47 morphants are shown relative to zic3 control morphants and uninjected controls. (d) Confirmation of exon 8 deletion in pfkp SB morphants by RT-PCR targeting the exon 8–intron 8 splice junction. Upper schematic: small horizontal arrows denote primer annealing sites. The horizontal line without an arrowhead denotes the SB morpholino target site. The severely truncated exon 8 (see §3) is represented by cross-hatching. Lower panel (RT-PCR): large arrowhead denotes the pfkp deletion fragment. SB, pfkp splice block. (Online version in colour.)