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. 2016 Oct 26;5:e19799. doi: 10.7554/eLife.19799

Figure 5. Inhibition of β-catenin degradation promotes progression from G1 into S/G2 following mechanical strain.

Figure 5.

(A) Distribution of mAG-Geminin in Fucci MDCK monolayers treated with DMSO, D4476, or D4476 and iCRT3 0 hr or 24 hr after the application of No Strain or High Strain (~8.5%) using the biaxial live cell stretcher. Scale bar: 150 μm. Quantification of percent cells Geminin positive in Fucci-MDCK monolayers after No Strain (B) or High Strain (15%) (C); percent cells Geminin positive in D4476 treated monolayers are statistically significant (p<0.05) relative to DMSO monolayers following mechanical strain at 16–24 hr. (D) Single cell tracking quantification of number of cell objects accumulated in S/G2 (red to green fluorescence, left) during 24 hr. All quantifications were from 2–4 independent experiments and included analysis of at least 15,000 cells. Quantifications were mean +/- SEM; unpaired t-test (B, C) or Kolmogorov-Smirnoff (D) test p values<0.01 (**), and <0.001 (***).

DOI: http://dx.doi.org/10.7554/eLife.19799.021

Figure 5—source data 1. Data used to construct graphs in Figure 5.
DOI: http://dx.doi.org/10.7554/eLife.19799.022
elife-19799-fig5-data1.xlsx (73.2KB, xlsx)
DOI: 10.7554/eLife.19799.022