Table 1.
Characteristics of 38 microsatellite loci developed in Lancea tibetica.
| Locus | Primer sequences (5′–3′) | Repeat motif | Fragment size (bp) | Ta (°C) | GenBank accession no. |
| LT4 | F: ATTGATTGATTCACGTTCCAAAT | (TA)6 | 132 | 54 | KU764519 |
| R: TGAAAATGAATAACTTGGGGATCT | |||||
| LT7 | F: TTTGGAAAGCATGATCTACCACT | (AAT)5 | 151 | 56 | KU764520 |
| R: TTTCTGGACTGTTGTAATCTTGAAA | |||||
| LT9 | F: GGATTTCTAAGTGCAATCCTCAA | (GA)7 | 140 | 51 | KU764521 |
| R: CATCACTCACCAAATGAAAGACA | |||||
| LT10 | F: AATTGTTCCAGGTATGCAGTGTT | (TG)6 | 155 | 51 | KU764522 |
| R: CTATTCTGCAAGTTAATGCAGGG | |||||
| LT12 | F: GTAGACATTTTTGCAGCACCTCT | (CAT)5 | 150 | 51 | KU764523 |
| R: ATGAGGACTCAAAGACAGCTCAG | |||||
| LT15 | F: CTTATAACCTATCGTTCTCCGGC | (AG)7 | 138 | 54 | KU764524 |
| R: ATTTCGCTCTCTCTTTCACACAC | |||||
| LT16 | F: TGTATTGTCAATGGAAGAGGCAT | (AAG)4 | 155 | 51 | KU764525 |
| R: GAATGAGATGCTCCACTAACCAC | |||||
| LT18 | F: AACAAGTTTATGCAAGGAGGAGA | (TCT)5 | 160 | 51 | KU764526 |
| R: CCCAAGTCCCAAATGATATAAAA | |||||
| LT25 | F: GATGCCAAGGAATTGTTATATGC | (TA)7 | 153 | 51 | KU764527 |
| R: TTTCTAGAAGTCGGAGCTGTCC | |||||
| LT28 | F: AACAGCAATGGCAATATGGTATC | (TA)9 | 158 | 56 | KU764528 |
| R: AACTGTTCAAGTTGGCAAAACAT | |||||
| LT6 | F: TCTATCGGTGCTAAAACACCTTC | (GT)11 | 153 | 51 | KX377923 |
| R: CTCATCCTCATCATACCGATCAT | |||||
| LT11 | F: TTGCCCTTATGTTTATCAAGGAA | (CT)6 | 144 | 51 | KX377922 |
| R: CACAGAAGAAGGATGAGGAGAAA | |||||
| LT17 | F: GACAGAACCCCTCTCTGAATCTT | (TAC)4 | 152 | 51 | KX377921 |
| R: GCGCCATAAGGTATAGCACTTC | |||||
| LT19 | F: ATTACCAACTTTCAACCAAAGCA | (CCT)4 | 159 | 51 | KX377920 |
| R: GCTTGTTGTCTTCTTTCCCAATA | |||||
| LT26 | F: TGAGCAGGTGCCTTTATTGTTAT | (CT)7 | 149 | 58 | KX377919 |
| R: TCAGCAGATCCTTATTATTTGTGC | |||||
| LT30 | F: AGGTCAGGAACAACAATACCTCA | (AG)8 | 158 | 49 | KX377918 |
| R: CTATATATCTTGCTTGCAAATCCG | |||||
| LT40 | F: TCTCTCTTTCTTCCCTCTCCATC | (GA)10 | 148 | 55 | KX377917 |
| R: AAATCAAGGAATCTGTGCAATGT | |||||
| LT45 | F: GGAGAGGGAAAAGAAGAAGAAGA | (GAA)4 | 78 | 53 | KX377916 |
| R: TACCAATGTAGCCGGAAATAAAG | |||||
| LT49 | F: AACGAAAATACTTTCCGTCTACAAA | (AT)8 | 121 | 52 | KX377915 |
| R: CTTGTTCTGGTCTGGTTTAAGGA | |||||
| LT60 | F: CTATAAATACCTCCCTCCCCCTC | (CT)7 | 127 | 51 | KX377914 |
| R: GTTTACGAGCACTCCTAGGTGAA | |||||
| LT61 | F: TGCCTATTCTTTACAAGAGCACA | (AT)6 | 117 | 52 | KX377913 |
| R: TTAATTGTAAATCGCAAAAACCC | |||||
| LT65 | F: TAAATGGTTTGCATCTTGGAAAT | (TAT)5 | 119 | 51 | KX377912 |
| R: GCAAAAATAAGTTTAACCGCGTA | |||||
| LT66 | F: TTTTGCTTTGTTGGATTCTTGAT | (GAT)4 | 148 | 53 | KX377911 |
| R: GCATCCTAAACTTACCGTTTTCA | |||||
| LT67 | F: TTTTGCAGGTTTAAGACAAAGGA | (GT)7 | 106 | 51 | KX377910 |
| R: TACATCGACACTTTTCAATCCAA | |||||
| LT69 | F: AGCGTAAGAAGATGATAGAAGGG | (AAT)4 | 145 | 51 | KX377909 |
| R: TGATCCTATTAGAGTTGCAAACG | |||||
| LT72 | F: TCAAACAAGCATGGGAGTACTTT | (AGA)4 | 140 | 52 | KX377908 |
| R: TTTGAACGAATTAGAGGAGGACA | |||||
| LT75 | F: ATACCAACCTGTGGCGTATATTG | (AT)6 | 150 | 51 | KX377907 |
| R: TGAAGATGTAAGAACAACCAGCA | |||||
| LT77 | F: GATCATGTCCCATCAAATTCAAC | (AT)14 | 157 | 51 | KX377906 |
| R: TTGTGTTATCTCCTGCGGTACTC | |||||
| LT79 | F: GAAGAGGTCAAGGCAAAGATACA | (AG)6 | 144 | 51 | KX377905 |
| R: TGAAATCGAGAATTGAAGAACAAA | |||||
| LT81 | F: TAGAAAAGTGAGGAATGGGACAA | (AT)6 | 157 | 50 | KX377904 |
| R: TGGTTTAGGAAATTTAACGATTGA | |||||
| LT83 | F: CATAATTTTGTGAGATCTTGGGC | (TTG)4 | 145 | 52 | KX377903 |
| R: AATTCTCCAAATGCAGATGATGT | |||||
| LT86 | F: TACTTGCTCCCCAAGTCTTCATA | (AG)8 | 135 | 51 | KX377902 |
| R: CGAGTGTAAGGCGTTAGGAGATA | |||||
| LT87 | F: AAGTACTCGAGAAGCAGGAGTCA | (TTA)4 | 116 | 49 | KX377901 |
| R: CCACCATAAAATCCTTTCCAAAT | |||||
| LT91 | F: GTACTTAGCGTGGGACTTTGTTG | (TGA)7 | 143 | 50 | KX377900 |
| R: CCACATCATCATCAATTGCATAC | |||||
| LT93 | F: AGCCAGTCGTCTCATTACAAAAA | (CT)6 | 138 | 55 | KX377899 |
| R: TTCTGCAGAGACTGGATCTGAAT | |||||
| LT95 | F: CGCAGTAGCAGATAGTGAATGTG | (ATC)5 | 119 | 54 | KX3779898 |
| R: TCCTCAAAATCAATGTCAGTGTG | |||||
| LT97 | F: TCGGGTTTATGTCTTACACTTGAG | (AAT)4 | 148 | 53 | KX3779897 |
| R: AGATCCTTAATTTTTATGAGCAATCA | |||||
| LT98 | F: ACATTGAAGACTAAGACATGGCG | (GA)6 | 156 | 52 | KX3779896 |
| R: GAGATACAAACCCTAACCCTCGT |
Note: Ta = annealing temperature.