Effect of diet on Pgp gene expression in wild type mice. The expression of Abcb1a, the isoform of Pgp expressed in liver and intestinal cells, was measured in (A) jejunum samples and (B) liver samples isolated from mice on each of the four diets by RT-qPCR. NFNC diet (25% kcal fat + 0.02% w/w cholesterol), HFNC diet (45% kcal fat + 0.02% w/w cholesterol), NFHC diet (25%kcal fat + 0.20% w/w cholesterol), HFHC diet (45% kcal fat + 0.20% w/w cholesterol). The mean relative expression is shown ± SD, n=9–10. *p<0.05 vs. NFNC by one-way ANOVA and Tukey post-hoc tests