Effect of P-glycoprotein deletion on lipoprotein cholesterol. Equal volumes of pooled serum were separated by fast protein liquid chromatography. Each panel contains the total cholesterol concentration in the fractions obtained from wild-type FVB mice (WT: hollow markers) or Pgp knockout mice (KO: grey markers) administered the following test diets: (A) NFNC (25% kcal fat + 0.02% w/w cholesterol), (B) HFNC (45% kcal fat + 0.02% w/w cholesterol), (C) NFHC (25%kcal fat + 0.20% w/w cholesterol), (D) HFHC (45% kcal fat + 0.20% w/w cholesterol). Pools consisted of equal volume aliquots from 6–10 mice. The cholesterol was distributed into VLDL, LDL/sub fraction 1 of HDL (HDL1), and HDL.