Table 5.

Effects of diet on the expression of FXR-target genes in the liver of wild type and Pgp knockout mice.

Genotype	Gene	0.02% (w/w) cholesterol						0.20% (w/w) cholesterol
		25% kcal fat			45% kcal fat			25% kcal fat			45% kcal fat
		Mean	±	SD	Mean	±	SD	Mean	±	SD	Mean	±	SD
Wild type	Abcb4	1.00	±	0.23	*1.68	±	0.35	*1.39	±	0.24	*1.65	±	0.28
	Abcb11	1.00	±	0.29	1.06	±	0.36	0.95	±	0.21	1.00	±	0.22
	Mrp2	1.00	±	0.79	1.50	±	1.09	1.13	±	1.37	1.98	±	2.29
	Shp	1.00	±	1.22	2.31	±	2.1	1.24	±	1.25	4.34	±	4.67
	Slc27a	1.00	±	0.82	1.24	±	1.25	1.91	±	1.45	1.79	±	0.66
Pgp knockout	Abcb4	0.90	±	0.27	*1.88	±	0.44	*1.58	±	0.32	1.12	±	0.24
	Abcb11	0.76	±	0.23	*1.41	±	0.49	0.65	±	0.17	*1.14	±	0.35
	Mrp2	0.76	±	0.99	*0.97	±	1.52	0.69	±	0.54	4.65	±	5.24
	Shp	0.67	±	0.66	0.44	±	0.59	1.26	±	1.09	*3.22	±	3.58
	Slc27a	0.63	±	1.05	0.66	±	1.09	0.53	±	0.55	*1.69	±	1.14

Gene expression was determined by semi-quantitative RT-qPCR from RNA isolated from frozen liver samples. The mean ΔCt value of the WT-NFNC dietary group was set as the reference to which all samples were compared using the ΔΔCt methodology. The mean relative expression and SD is shown to indicate the variability of the response to dietary stimulus.

^*p<0.05 vs. NFNC diet by one-way ANOVA with Tukey post-hoc tests, n=8–10.