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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Eur J Cancer Prev. 2017 Jul;26(4):301–308. doi: 10.1097/CEJ.0000000000000263

Figure 4. Mouse mitochondrial respiration associated proton leak is enhanced by fish oil and fermentable fiber feeding in the proximal colon.

Figure 4

Following consumption of the experimental diets for 4 wks, isolated distal and proximal colonic crypts were immediately cultured and mitochondrial bioenergetic profiles were measured using the Seahorse XF24 Flux system as described in the Methods section. Proton leak is defined as the OCR attributed to all processes which allow ion movement across the mitochondrial inner membrane, and was measured as the difference in OCR following oligomycin and rotenone challenge. Data are normalized to the original basal OCR level (before challenging with mitochondrial inhibitors) (Mean ± SE, n=15). Means not sharing a common letter are significantly different (P < 0.05).