Figure 6.

The Mevalonate Pathway-Mediated Protein Prenylation Is Required for Normal Cell Size Scaling of Mitochondrial Functions

(A) Schematic of the mevalonate pathway. Chemical inhibitors used are shown in red. ^∗Note that Drosophila cells are incapable of de novo cholesterol synthesis.

(B) Mevalonate pathway regulates cell size scaling of rΔΨm through protein prenylation. Cell size scaling of rΔΨm in Kc167 cells treated with pitavastatin and indicated metabolites for 72 hr. n > 2 × 10⁵ cells.

(C) Cell size scaling of rΔΨm variability after 72 hr of treatment with statin. The statin effects were fully rescued by GGPP. Arrows indicate the cell size with the minimum variability. n > 2 × 10⁵ cells.

(D) Cell size scaling of respiration in control Kc167 cells. The experiment carried out as in Figure 1E. Data represent mean ± SD (n = 4).

(E) Same as (D), but cells were treated with statin for 72 hr before the oxygen consumption assay. Data represent mean ± SD (n = 2–4).

See also Figure S6.