Table 2.

Primer sequences for sequencing of TP53 exon 4–10.

Exon		Primer sequence	Fragment (bp)
4	F-N1	5′-CCATGGGACTGACTTTCTGC	534
	R-N1	5′-GAGGAATCCCAAAGTTCCAA
	F-N2	5′-CTGGTAAGGACAAGGGTTGG	457
	R-N2	5′-AGAAATGCAGGGGGATACG
5 + 6	F-N1	5′-GGAGGTGCTTACGCATGTTT	588
	R-N1	5′-GGGAGGTCAAATAAGCAGCA
	F-N2	5′-GCCGTCTTCCAGTTGCTTTA	506
	R-N2	5′-GCCACTGACAACCACCCTTA
7	F-N1	5′-CCTGCTTGCCACAGGTCT	294
	R-N1	5′-TGATGAGAGGTGGATGGGTAG
	F-N2	5′-TGCTTGCCACAGGTCTCC	236
	R-N2	5′-GGTCAGAGGCAAGCAGAGG
8 + 9	F-N1	5′-GGGAGTAGATGGAGCCTGGT	486
	R-N1	5′-CCCCAATTGCAGGTAAAACA
	F-N2	5′-GGGACAGGTAGGACCTGATTT	431
	R-N2	5′-AAGAAAACGGCATTTTGAGTG
10	F-N1	5′-TGCATGTTGCTTTTGTACCG	300
	R-N1	5′-GAAGGCAGGATGAGAATGGA
	F-N2	5′-TGCATGTTGCTTTTGTACCG	263
	R-N2	5′-CCTAGGAAGGCAGGGGAGTA

The nest 2 primers contain T3 and T7 tags on F-N2 and R-N2, respectively.