Table 1.

Total number of pseudogenes and full open reading frames (ORFs) of α-gliadin sequences obtained from 11 contrasted spelt accessions, distribution of the full-ORF sequences and enumeration of the canonical forms of the four T-cell stimulatory epitopes among each genome and each accession.

Name*	Accession number	Total number of sequences	Pseudogenes	Full ORF	A	B	D	DQ2.5-glia-α1				DQ2.5-glia-α2				DQ2.5-glia-α3				DQ8-glia-α1
								A	B	D	TOT	A	B	D	TOT	A	B	D	TOT	A	B	D	TOT
BEL08	PI348315	42	2	40	11	15	14	9	0	16	25	0	0	16	16	9	0	14	23	0	0	3	3
DK01	PI361811	42	0	42	13	19	10	13	0	13	26	0	0	13	13	10	0	7	17	0	0	5	5
SPA03	PI348572	41	1	40	26	8	6	25	0	12	37	0	0	12	12	24	0	6	30	0	0	4	4
BUL04	PI295063	45	2	43	17	15	11	15	0	17	32	0	0	17	17	15	0	9	24	4	0	4	8
GER11	PI348114	41	2	39	13	17	9	13	0	11	24	0	0	11	11	12	0	7	19	1	0	3	4
GER12	PI348120	36	1	35	18	9	8	16	0	10	26	0	0	10	10	16	0	8	24	1	4	2	7
TAD06	K 52437	47	1	46	14	27	5	8	0	7	15	0	0	7	7	14	0	5	19	0	0	1	1
SWI23	PI347939	45	4	41	15	17	9	15	0	12	27	0	0	12	12	15	0	7	22	0	2	2	4
US06	PI355595	38	4	34	21	7	6	14	0	11	25	0	0	11	11	21	0	6	27	0	1	5	6
Iran77d	CGN 06533	45	1	44	22	7	15	12	0	22	34	0	0	22	22	22	0	14	36	0	0	8	8
IRA03	CGN12270	42	0	42	15	11	16	10	0	24	34	0	0	26	26	15	0	10	25	0	0	11	11
		464	18	446	185	152	109	150	0	155	305	0	0	157	157	173	0	93	266	6	7	48	61

The 446 full-ORF α-gliadin expressed sequences were checked for the presence of the four T-cell stimulatory epitopes in their canonical forms: P{F/Y}PQPQLPY (DQ2.5-glia-α1), PQPQLPYPQ (DQ2.5-glia-α2), FRPQQPYPQ (DQ2.5-glia-α3) and QGSFQPSQQ (DQ8-glia-α1)

*: Names used for the same accessions in Bertin et al. (2004) except for Iran77d, which was named as in Dvorak et al. (2012)