Fig. 3.

Integrals of discriminatory metabolites identified by the ¹H-NMR spectroscopic analysis of hepatic extracts after APAP/AMAP administration (A). Drug metabolite ratios of APAP and AMAP conjugates to their respective parent molecules in the hepatic extract and urinary metabolic profiles at the 1-h time-point are also shown (B). Kruskal–Wallis test coupled to Dunn’s multiple test correction was used to calculate the statistical significance of endogenous metabolic perturbations, which is indicated by the asterisks, * and ** (p < 0.05 and p < 0.01, respectively). Key -SG, glutathionyl conjugate; -S, sulfate conjugate; -G, glucuronide conjugate; -Cys, cysteinyl conjugate; -NAC, N-acetylcysteinyl conjugate