Fig. 3.

Impact of treatment of human-derived buccal cells (line TR-146) with synthetic cannabinoids on formation of oxidatively damaged purines (FPG-sensitive sites, a, b) and pyrimidines (Endo III sensitive sites, c, d). The cells were exposed to the drugs for 24 h. Subsequently, the nuclei were isolated and treated with enzymes or with the buffers only as described in the “Materials and methods” section. Subsequently, DNA migration was monitored. Bars indicate means ± SDs of results obtained with two cultures per experimental point in a representative experiment (values which were obtained with the enzyme buffers were subtracted from values which were obtained with the enzymes). From each culture, two slides were made and 50 cells were evaluated per slide