Fig. 4.

Generation of tyrosinase mutant zebrafish by CRISPR/Cas9. (A) Two alleles of F1 heterozygous mutants (+18, +1 insertion) were acquired. (B) F1 heterozygous mutants were confirmed using T7 endonuclease I. (C) Homozygous mutant F2 generation shows shifted band. (D) WT protein consists of SP, EGF-like Domain, CuA, CuB, TM. KO #2 mutants fish (+1) showed one base insertion at base 78 in exon 1 (c.78/79 insT, 26^th amino acid), which generated premature stop at 98^th amino acid. SP - Signal peptide, EGF-like domain - Epidermal growth factor-like domain, CuA, CuB - Copper binding domain A, B, TM - Transmembrane domain.