FIGURE 5.

Reverse transcription polymerase chain reaction (RT-PCR) validation of DEGs identified by RNA-Seq. Expression pattern of 14 DEGs was validated by RT-PCR. For the RT-PCR experiments C7 and T7 represent cDNA templates synthesized from RNA samples at 7 dpt from control and treatment samples respectively. RT-PCR was performed with intron-spanning primers of the different genes in at least three biological replicates for all the samples. Cyclophilin and Actin were used as internal reference genes.