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. 2016 Sep 21;291(46):23895–23905. doi: 10.1074/jbc.M116.728550

FIGURE 6.

FIGURE 6.

PTP-MEG2 inhibits NGF/TrkA signaling-dependent neurite outgrowth in PC12 cells. A, immunoblot showing persistent overexpression of PTP-MEG2:WT or PTP-MEG2:CS mutant in cells transduced with the respective PTP-MEG2 lentiviruses and a lentivirus expressing TrkA-eGFP in comparison with control cells transduced only with the virus expressing TrkA-eGFP. Cell lysates were prepared on day 5 of NGF treatment. Molecular mass standards are indicated on the left. B, epifluorescence microscopy images of neurite outgrowth in representative differentiated cells on day 5 of NGF treatment under each indicated condition. Transduced cells were identified by the expression of TrkA-eGFP. Scale bar = 200 μm. C, the percentage of differentiated cells from over 200 transduced cells under each indicated condition upon NGF treatment over 5 days (*, p < 0.05; ***, p < 0.001; chi-square analysis compared with control each day and adjusted for multiple comparisons). The results were reproducible in three independent experiments. D, the total neurite length of individual differentiated cells (n = 19–20) on day 5 of NGF treatment under each indicated condition (*, p < 0.05, one-way ANOVA with multiple comparisons).