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The ΔpvdN mutation does not affect iron acquisition under iron-limiting conditions at pH values that allow growth of strain A506. P. fluorescens (WT and ΔpvdN) was grown under iron-limiting (CAA) and iron starvation (CAA + EDDHA) conditions for 36 h at the indicated pH values, colonies were imaged (left), and the pyoverdine fluorescence was detected (right). Note that there is no detectable difference in growth and pyoverdine formation between the two strains.
