TABLE 1.
Primers used in this study
| Name | Sequence | Restriction site | Purpose |
|---|---|---|---|
| PfA506-PvdN-F1-MR | CTGTTCGGATCCCGAGCATGAGGTGCGCTATAAGATCATCTCC | BamHI | Forward primer for pvdN left flanking region |
| PfA506-PvdN-F2-MR | CTAACCACCACGAAGTAAGTCCATGACCCGGTTATGACCCCTTTTCCCTTTGAACGAGAC | Forward primer for pvdN right flanking region | |
| PfA506-PvdN-R1-MR | GGTCATGGACTTACTTCGTGGTGGTTAG | Reverse primer for pvdN left flanking region | |
| PfA506-PvdN-R2-MR | GCATCTAAGCTTTGGCCAAACAAGGCTGAACCCTGTAGGAG | HindIII | Reverse primer for pvdN right flanking region |
| PfA506-PvdN-DF-MR | AACGGCGATAACGCTCCGCACAAAC | pvdN genomic deletion control primer | |
| PfA506-PvdN-DR-MR | ACGACGTGCTCAAGACCGAGATTTC | pvdN genomic deletion control primer | |
| PfA506-PvdN-F-MR | TTCGACCATATGACCGACCGCCGTACATTTCTCAAGCAGG | NdeI | Forward primer for cloning PvdN-coding region into pEXH5 |
| PfA506-PvdN-strep-R-MR | CCGATTAAGCTTTTACTTTTCGAACTGCGGGTGGCTCCATAACCGCTGGCTCAGCAGGGTCATGAAG | HindIII | Reverse primer for cloning PvdN-coding region into pEXH5 |
| PfA506-PvdP-DF-MR | GTTGCAGTTCCTTGTCGCTGAGGTTG | pvdP genomic deletion control primer | |
| PfA506-PvdP-DR-MR | CCGGCCAGCATTTCTGGCTATTC | pvdP genomic deletion control primer | |
| PfA506-PvdP-F1-MR | TGCTCTAAGCTTTTGGTCGACATCTGCGACACGTCGATGATCAC | HindIII | Forward primer for pvdP left flanking region |
| PfA506-PvdP-F2-MR | ACGCTTTAAAACGTGTTGCAGGCGCTCTAGG | Forward primer for pvdP right flanking region | |
| PfA506-PvdP-R1-MR | GCGCCTGCAACACGTTTTAAAGCGTTGTCATTGGCTACCTTAGGAAACGGC | Reverse primer for pvdP left flanking region | |
| PfA506-PvdP-R2-MR | TTAAACCCCGGGAGGTACTGGGTAGCCTGTCCAGGCGTATCGACGAG | XmaI | Reverse primer for pvdP right flanking region |
| PfA506-PvdN_A261-F-MR | CTTCTTCATCGCTGGCACCCACGCGTGGATGTTCGGCCCGCGGGGCACCGGCC | Primer for K261A exchange by oePCR | |
| PfA506-PvdN_A261-R-MR | CGCGTGGGTGCCAGCGATGAAGAAGTCGCAGTG | Primer for K261A exchange by oePCR | |
| PfA506-matPvdN-F-MR | ATACGGCATATGGCCGCCACGAACGACCCATGGACGGGATTGAAACAAC | NdeI | Forward primer for cloning mature PvdN-coding region into pEXH5 |
| pEXH5-RBS-F-MR | GGCGCGGGATCCGTTTAACTTTAAGAAGGAGATATAC | EcoRI | Forward primer for subcloning from pEXH5 into pME6010 |
| pEXPT7-strep-term-R-MR | CCCCTTGAATTCAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTTTACTTTTCGAACTGCGGGTGGCTCC | BamHI | Reverse primer for subcloning from pEXH5 into pME6010 |