TABLE 2.
SAXS structural parameters
| Rga | Rgb | I(0)a | I(0)b | Dmaxc | Vpd | MMe | MMf | MMg | Oligomeric state | |
|---|---|---|---|---|---|---|---|---|---|---|
| Å | Å | a.u. | a.u. | Å | Å3 | kDa | kDa | kDa | ||
| Ero1α-AAh | 26.6 ± 0.1 | 26.54 ± 0.05 | 17.75 ± 0.03 | 17.68 ± 0.02 | 96 | 9.99 × 104 | 55.2 | 60.5 | 54.3 | Monomer |
| Ero1α-AASSh | 26.2 ± 0.1 | 26.19 ± 0.05 | 17.49 ± 0.03 | 17.46 ± 0.02 | 92 | 1.02 × 105 | 54.4 | 61.8 | 54.3 | Monomer |
| BSAi | 27.7 | NDj | 21.35 ± 0.01 | ND | ND | ND | ND | ND | 66.4 | Monomer |
a Guinier analysis using the Q range from 0.01562 to Qmax <1.3/Rg.
b Estimates in real space upon P(r) determination.
c Maximum dimension estimated using the GNOM package.
d Porod volume.
e Molecular mass calculated using the I(0) value for BSA as the standard.
f Molecular mass calculated according to the empirical relationship MM = VP/1.65 (42).
g Theoretical molecular mass calculated according to amino acid sequences.
h Extrapolated to infinite dilution.
i At a concentration of 2.75 mg/ml (39).
j ND, not determined.