FIGURE 7.

Rotary torque of TA2F₁ is the highest determined by fluctuation theorem. A, typical time course of TA2F₁γ2c rotation with 2 mm ATPγS filmed at 5000 fps. Boxed in red are the stepping regions of the trace, θ(t), used to determine the torque. The steps were determined by eye. B and C, 10 TA2F₁ rotation traces were collectively analyzed. B, ln[P(Δθ)/P(−Δθ)] as a function of Δθ/k_BT. The red line shows the average torque of the 10 molecules. The slope revealed a rotary torque of 52.4 ± 4 pNnm/radian (rad) in the case of Δt = 6 ms. C, N_FT as a function of Δt for the recording rate of 5000 fps. Torque from 5 to 10 ms was regarded as consistent enough to judge the rotary torque of TA2F₁. deg, degrees. Error bars represent S.E.