TABLE 2.
Primer | Sequence (5′–3′)a | Description or mutations | Direction |
---|---|---|---|
Tg-cysF | ATCTGTTCTACTCCGGTGTGCG | 5′ external primer upstream from an AflII restriction site | Forward |
Tg-cysR | AGGAAACGCTGGATTTTGCGGG | 3′ external primer downstream from an AfeI restriction site | Reverse |
Tg-cysMutF | GAGGAGCGttgTCAGTCCAAAGAC | γH107C | Forward |
Tg-cysMutR | TGGACTGAcaaCGCTCCTCGATC | γH107C | Reverse |
Tg-cysMutF2 | CGAACCGGACtGTGAATCGGTC | γS210C | Forward |
Tg-cysMutR2 | GACCGATTCACaGTCCGGTTCG | γS210C | Reverse |
a Lowercase letters indicate specific changes in the primer for site-directed mutagenesis.