FIGURE 1.

Palmitate and D609 induce SM precursors in C2C12 myotubes. C2C12 myotubes were treated with BSA (control), BSA-conjugated palmitate (palmitate, 500 μm), and/or D609 (300 μm) for 16 h. Lipids were extracted and quantified by targeted lipidomics. A–E, data shown are the sum of SM or SM precursors with various acyl chain lengths present within detection limits (n ≥5). F, efficacy of D609 treatment in SMS activity was measured by accessing the amount of NBD-SM converted from NBD-C₆-ceramide (n ≥3); * and # denote significance of palmitate or D609, respectively, at p < 0.05.