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. 2016 Oct 3;291(46):23999–24008. doi: 10.1074/jbc.M116.749994

FIGURE 4.

FIGURE 4.

MukB DNA-binding activity is required for catenation. A, Topo III alone does not catenate the gDNA. Standard reaction mixtures containing gDNA and 25 nm Topo III with the indicated concentrations of Mg(OAc)2 were incubated for 30 min at 37 °C, processed, and analyzed as described under “Experimental Procedures.” The assay was repeated at least three times, a representative gel is shown. B, nitrocellulose filter DNA binding assays of wild type MukB and MukBdna. Binding to a duplex 5′-32P 50-bp long oligonucleotide was measured as described under “Experimental Procedures.” The mean ± S.D. of three experiments is plotted. C, MukB DNA-binding activity is required for DNA catenation. Standard reaction mixtures containing 25 nm Topo III and either no MukB or the indicated concentrations of either MukB or MukBdna were incubated for 30 min at 37 °C processed and analyzed as described under “Experimental Procedures.” The assay was repeated at least three times, a representative gel is shown.