FIGURE 10.

Egfl7 regulates the degradation of IκBα by the proteasome. A, HUVEC were transfected with siCtrl or siEgfl7, treated 4 days later with 10 ng/ml TNFα, and lysed at the indicated time points. The cell extracts were prepared, and proteins (10–20 μg) were analyzed by Western blotting using antibodies against the indicated proteins (left). The results are representative of three experiments. B, HUVEC were transfected with siCtrl or siEgfl7 and treated 4 days later with the proteasome inhibitor MG132 for 2 h prior to adding 10 ng/ml TNFα for the indicated time. The cells were lysed, and proteins (5 μg) were analyzed by Western blotting against total IκBα or GAPDH. The results are representative of two experiments.