Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 20;291(46):24017–24028. doi: 10.1074/jbc.M116.731331

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Specific targeting and rescue of egfl7 do not affect expression of the miR-126 locus. A, HUVEC were transfected with siCtrl (white) or with two different siRNA targeting Egfl7: siEgfl7 #9 (black) or siEgfl7 #10 (gray) and cultured for 4 days. Left panel, total RNA were prepared and analyzed by duplex RT-qPCR for Egfl7 expression. ***, p < 0.001. Middle panel, protein extracts were prepared and analyzed for the presence of Egfl7 or actin by 12% SDS-PAGE and Western blotting. Right panel, HUVEC were treated as in A, and analysis of miR126-3p and miR126-5p expression was performed by RT-qPCR, using the U6 snRNA as normalizer. B, HUVEC were transfected with siCtrl or siEgfl7#10 as in A and then with pcDNA3 (pCtrl) or a pcDNA3-human Egfl7 expression plasmid (pEgfl7). Left panel, RT-qPCR analysis of Egfl7 expression on triplicate experimental samples. Right panel, RT-qPCR analysis of expression of miR126-3p and of miR126-5p in pCtrl- or in pEgfl7-transfected HUVEC on triplicate experimental samples. ***, p < 0.001; ns, non-significant.