FIGURE 8.

Egfl7 prevents the activation of the NF-κB pathway in endothelial cells. A, HUVEC were transfected with siCtrl or siEgfl7, treated 4 days later with 10 ng/ml TNFα, and lysed at the indicated time points. The cell extracts were prepared, and proteins (10–20 μg) were analyzed by Western blotting using antibodies against the indicated proteins (left). The results are representative of three experiments. B, membranes were exposed to a Las3000 system, and intensities were quantified using the Multigauge V3.0 software.