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Egfl7 does not affect the IKK complex. HUVEC were transfected with siCtrl or siEgfl7, treated 4 days later with 10 ng/ml TNFα, and lysed at the indicated time points. Cell extracts were prepared, and proteins (10–20 μg) were analyzed by Western blotting using antibodies against the indicated proteins (left). The results are representative of three experiments.
