TABLE 1.
Template strand sequence is shown. The extended −10 element is underlined and the +1 nucleotide is shown in bold
| Oligo primers | Sequence |
|---|---|
| spo0B (−105) F | AAGAATTCGTTCTGCCTGGCTGCAAATC |
| spo0B (−105 KpnI) F | AAGGTACCGTTCTGCCTGGCTGCAAATC |
| spo0B (+49) R | AAGGATCCCGCACTCCCAATCATTTAATTTC |
| spo0B (+30) R | TTTCTTATTTAGGAGTCTGTATAAGTGTG |
| spo0B (+12) R | AAGGATCCGTATAAGTGTGTATTATGAATTATATCAGAGAAG |
| spoB mut1 F | GGGAAAAACAATTGTTGATCTAACAAAGCC |
| spoB mut1 R | GGCTTTGTTAGATCAACAATTGTTTTTCCC |
| spoB mut2 F | GGGACCCACAATTGTTGATCTAACAAAGCC |
| spoB mut2 R | GGCTTTGTTAGATCAACAATTGTGGGTCCC |
| spo0B RT F | GGCCATTCCCGGCATGATTGGATGAATAAG |
| spo0B RT R | GGATCGGCAAAGGCGCCGTGAAAATCAAGG |
| rpoA F | CTTCATATCGCGACTCTTGGTGAGAATGCG |
| rpoA R | GATCCAATTCTTCAATTGTCATTTC |
| rpoE F | AAGATATCATGGGGAGTGTCCGACCATGGGTATCAAAC |
| rpoE R | AAGGTACCCTATATTTAATTTCCTCTTCTTCATCATC |
| rpoEL51C F | GTGAAAAAAGAAGAGTGTGGAGACCGC |
| rpoEL51C R | GCGGTCTCCACACTCTTCTTTTTTCAC |
| rpoD F | GGATCCATGGCTGATAAACCCACG |
| rpoD R | GAATTCAAGCTTTTATTCAAGGAAATCTTTCAAACGTTTACTTC |
| rpoDΔ4.2 F | GACGGCCGTACATGAACATTAGAAG |
| rpoDΔ4.2 R | CTTCTAATGTTCATGTACGGCCGTC |
| DNA | |
| spo0B DNA template | AAGAATTCGTTCTGCCTGGCTGCAAATCAACCAAAGCCAGAAAAAAGGAACATGATATTTCTGGGAAAAACAATTGTTTTTCTAACAAAGCCTTCTCTGTTATAATTCATAATACACACTTATACAGACTCCTAAATAAGAAATTAAATGATTGGGAGTGCGGGATCCTT |