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. 2016 Sep 12;291(46):24133–24147. doi: 10.1074/jbc.M116.748236

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Extracellular domains of CNTNAP2 and CNTN2. A, SDS-PAGE analysis of purified recombinant CNTNAP2 (lane 1) and CNTN2 (lane 3). Markers (in kDa) are shown in lane 2. B, size exclusion chromatography of CNTNAP2; C, size exclusion chromatography of CNTN2; D, size exclusion chromatography of standards (2,000, 200, 66, 29, and 12.4 kDa) and resulting calibration line. Samples and standards were run in triplicate. The average elution volume (EV_ave) and S.D. value are shown. The apparent molecular masses (M_app) are indicated and reflect large versus small species.