TABLE 1.
Substrate specificity of the purified β-glycosidase GlyA1 and truncated GlyA1-ΔCt
| Substrate | Specific activity |
|
|---|---|---|
| GlyA1 | GlyA1-ΔCt | |
| units/g | ||
| pNPβGlc | 2226.8 ± 120.2 | 197.0 ± 10.6 |
| pNPβXyl | 2876.2 ± 111.1 | 624.3 ± 34.1 |
| pNPβXylb | 301.6 ± 8.3 | 14.8 ± 0.70 |
| pNPβCel | 290.7 ± 9.9 | 17.04 ± 0.50 |
| pNPβGal | 11.8 ± 2.2 | 0.64 ± 0.01 |
| pNPβFuc | 1.18 ± 0.01 | 0.62 ± 0.01 |
| pNPαAraf | 1.16 ± 0.01 | 0.64 ± 0.01 |
| pNPαArap | 0.66 ± 0.01 | 0.33 ± 0.01 |
| Cellobiose | 551.5 ± 2.6 | 43.6 ± 2.2 |
| Cellotriose | 532.6 ± 0.5 | 44.4 ± 4.9 |
| Cellotetraose | 569.3 ± 0.4 | 48.7 ± 5.1 |
| Cellopentaose | 641.5 ± 0.5 | 53.3 ± 2.1 |
| Xylobiose | 634.2 ± 4.3 | 115.0 ± 4.4 |
| Xylotriose | 668.3 ± 7.2 | 136.1 ± 6.7 |
| Xylotetraose | 674.5 ± 11.5 | 174.7 ± 4.5 |
| Xylopentaose | 747.9 ± 3.5 | 196.4 ± 8.6 |
| Gentibiose | 535.2 ± 0.7 | 45.9 ± 1.2 |
| Sophorose | 602.8 ± 0.7 | 54.3 ± 4.5 |
| Lichenan | 68.6 ± 4.8 | 9.8 ± 0.7 |