TABLE 2.

Kinetic parameters of the purified β-glycosidase GlyA₁

Substrate	Km	kcat	kcat/Km
	mm	s−1	s−1m−1
pNPβGlc	10.7 ± 2.0	1.63 ± 0.38	152.3
pNPβXyl	8.8 ± 0.5	0.95 ± 0.44	107.8
pNPβCel	1.4 ± 0.2	0.51 ± 0.15	314.2
pNPβXylb	2.5 ± 0.3	0.73 ± 0.12	292.0
pNPβGal	7.6 ± 0.1	0.13 ± 0.04	17.1
pNPβFuc	4.8 ± 0.3	0.05 ± 0.01	10.4
pNPαAraf	7.8 ± 1.7	0.03 ± 0.01	3.85
pNPαArap	10.7 ± 3.4	0.01 ± 0.01	0.93
Celobiose	2.4 ± 0.3	0.07 ± 0.01	28.2
Xylobiose	4.7 ± 0.2	0.05 ± 0.01	10.6